Marine protected areas (MPAs) cannot be managed outside the context of human societies that are dependent on their associated ecosystems and resources. This means that local people’s perceptions need to be considered in the establishment of MPAs as well as their subsequent management, planning and decision making processes. Accordingly, this study investigated respondents’ perceptions of the Ponta do Ouro – Kosi Bay MPA. The MPA is part of the now proclaimed Lubombo Trans-frontier Conservation Area (TFCA). An interviewer – administered questionnaire was used to obtain primary data from 35 respondents, all resident in the study area and who are involved in various activities based on the coastal area and its marine resources. The focus of the study was on awareness regarding the establishment, impacts of the MPA, the setting of priorities for the MPA and lastly, respondents’ roles and responsibilities The findings from the study reveal low levels of awareness of the establishment of the MPA among respondents, although there was acknowledgement of its potential contribution to biodiversity conservation. Various types of impacts of the establishment of the MPA were noted. The establishment of the MPA was perceived to negatively impact on the access to, and use of, marine resources. It was also felt that the MPA would impact on the exercise of traditional authority.