No-take Marine Protected Areas (Nt-MPAs) constitute an indispensable tool for biodiversity conservation. Nevertheless, there are other instruments such as marine coastal co-management policy frameworks which may be also considered as tools for conservation or as ancillary conservation instruments. Using focus groups, semi-structured interviews and survey questionnaires the authors analysed small-scale artisanal fishers’ perceptions towards a coastal co-management regime in Chile and the potential to generate capacities and a social setting to scale-up marine conservation. Empirical evidence from the study shows artisanal fishers have indeed been empowered through the coastal co-management experience; however, there exist heterogeneity in their willingness to participate in the creation of Nt-MPAs, mainly determined by occupational mobility. Chilean artisanal fishers strongly support a bottom-up process in the conservation of marine biodiversity, though the need for top-down steering and guidance is also stressed, especially regarding enforcement.